Abstract
The genus Graphium belonging to order Microascales, comprises known wood pathogens that cause sapstain in timbers and wood degradation. However, this genus has been scarcely studied in Korea. Therefore, the current study was conducted to investigate the genus Graphium in freshwater environments as new habitat in Korea. Three strains, CNUFC PYW4-15, CNUFC BCW49, and CNUFC BCW48 were isolated from freshwater samples. Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS) and translation elongation factor-1 alpha (TEF-1α) gene sequences, the isolated strain, CNUFC PYW4-15 was identified as Graphium carbonarium as an unrecorded species in Korea. While the strains CNUFC BCW49 and CNUFC BCW48 were discovered as a new species, named Graphium aquaticum sp. nov.