Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis

过氧化物酶体增殖激活受体-γ 辅激活因子 1α 介导的通路是子宫内膜异位症的可能治疗靶点

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作者:Hisashi Kataoka, Taisuke Mori, Hiroyuki Okimura, Hiroshi Matsushima, Fumitake Ito, Akemi Koshiba, Yukiko Tanaka, Kanoko Akiyama, Eiko Maeda, Takuya Sugahara, Yosuke Tarumi, Izumi Kusuki, Khaleque N Khan, Jo Kitawaki

Methods

Ectopic endometriotic and eutopic endometrial stromal cells (SCs) were isolated and maintained in culture. PGC-1α was either overexpressed in the cells or knocked down using siRNA. The expression of PGC-1α and other factors during endometriosis was examined using real-time PCR and western blotting, cell proliferation was measured using Cell Counting Kit-8 (WST-8) assays and transcriptional activity was assessed using luciferase reporter assays. Main

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