LncRNA SNHG11 expression in acute myeloid leukemia patients and its relationship with the biology of acute myeloid leukemia cells

lncRNA SNHG11在急性髓系白血病患者中的表达及其与急性髓系白血病细胞生物学特性的关系

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Abstract

BACKGROUND AND OBJECTIVE: Acute myeloid leukemia (AML) is a malignancy involving the blood and bone marrow, marked by the uncontrolled growth and development of myeloid cells. LncRNA small nucleolar RNA host gene 11 (SNHG11) is aberrantly expressed in various cancers. Objective of current research was to explore the SNHG1 expression in AML patients and further explore its role and mechanisms in AML cell biology. METHODS: SNHG11 and miR-122-5p levels were detected by quantitative PCR. The diagnostic potential of SNHG11 was assessed via ROC curve analysis. Effects of SNHG11 on the 5-year overall survival (OS) of AML was detected by Kaplan-Meier (KM) curve. sh-SNHG11 was transfected into MOLM-13 cells to explore the growth and apoptosis of MOLM-13 cells. Cell growth and apoptosis were assessed using the CCK-8 kit and a flow cytometer. RESULTS: SNHG11 level was significantly elevated in AML patients. SNHG11 had diagnostic value for AML (AUC = 0.827, sensitivity = 72.58%, specificity = 79.74%). High-SNHG11 group had shorter OS than low group (P < 0.001, log-rank test). SNHG11 (HR = 2.036, 95% CI = 1.103-3.758) was a risk factor for poor AML prognosis. sh-SNHG11 markedly suppressed proliferation and increased apoptosis in MOLM-13 cells via miR-122-5p. CONCLUSION: SNHG11 could diagnose AML from healthy controls and related to poor prognosis of AML patients. SNHG11 could impact cell biology through miR-122-5p.

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