Assessment of distraction callus in rabbits by monitoring of the electrical impedance of bone

通过监测骨电阻抗评估兔骨牵引成骨痂

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Abstract

BACKGROUND AND PURPOSE: Evaluation of distraction callus is important for determination of the optimal time for removal of external fixation. We attempted to determine whether there might be a relationship between the electrical impedance of bone and callus maturation, with a view to using impedance as a way of knowing when to remove a fixator. METHODS: We applied an external lengthener to the right tibia of 24 rabbits and performed distraction at 1 mm/day for 10 days. Radiographs were taken and measurement of overall impedance between fixation pins was performed weekly after distraction. At weeks 2, 4, 6, and 8 after distraction (n = 6 each), resistivity of the bone to electrical conductivity was measured before killing. Cross-sectional area of the conduction pathway in callus and maximum bending stress were measured after excision of the tibia. RESULTS: The overall impedance increased statistically significantly from 1 to 6 weeks after completion of distraction. The resistivity of bone decreased over 4 weeks and the cross-sectional area of callus decreased significantly over 6 weeks, while the maximum bending stress increased significantly over the same time. We observed a negative correlation between the cross-sectional area of callus and maximum bending stress. INTERPRETATION: The impedance values, which are related to changes in electrical conductivity and the conduction pathway, increased due to the changes in the cross-sectional area of callus, despite the reduction in bone resistivity. Since the cross-sectional area of callus was correlated with maximum bending stress and the impedance values increased with the callus-remodeling process, we suggest that temporal increases in overall impedance reflect callus maturation.

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