Abstract
To investigate the effects of repeated sodium hypochlorite stress on the resistance of Salmonella enterica Serovar Enteritidis (S. Enteritidis) LWCC1051. LWCC1051 was exposed to Trypticase Soy Broth (TSB) containing sodium hypochlorite concentrations of 9 mmol/L, 10 mmol/L, and 11 mmol/L. After 13 repeated transfers and incubations, three sodium hypochlorite resisted LWCC1051 strains were obtained. The D-values and colony morphologies of these strains were assessed. Their survival rates at 60 °C, 65 °C, 70 °C, 75 °C, and - 20 °C were determined and lethality curves at these temperatures were fitted using the Weibull model. Additionally, the Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for these strains in various chemicals, including malic acid, citric acid, ascorbic acid, acetic acid, lactic acid, hydrochloric acid, sodium hydroxide, potassium hydroxide, calcium chloride, sodium chloride, and potassium chloride were ascertained. Sodium hypochlorite concentrations of 9 mmol/L, 10 mmol/L, and 11 mmol/L in TSB induced sodium hypochlorite resistance in S. Enteritidis. D-value increased with the frequency of stress exposure. Higher concentrations of sodium hypochlorite resulted in greater D-values and noticeable differences in colony morphologies. The Weibull model accurately represented the temperature resistance curves of LWCC1051 at the specified temperatures. With increasing sodium hypochlorite stress, both high and low-temperature resistances of LWCC1051 improved. Furthermore, under acetic acid stress, the MIC and MBC values of LWCC1051 strains, post exposure to 9 mmol/L, 10 mmol/L, and 11 mmol/L sodium hypochlorite, doubled. Sodium hypochlorite stress enhances the cross-resistance of LWCC1051 to high temperature, low temperature, and acetic acid treatments.