Abstract
Microbes can serve as mediators for the fabrication of complicated nano-structures, obviating the tedious and time-consuming methods of synthesis. The shape of a nanoparticle has a very prominent role in defining the functionality in prospective arenas. So, the flower shaped nanoparticles are in focus nowadays due to their enhanced electrocatalytic and optical properties as compared to the spherical ones. We present the biosynthesis of flower shaped gold nanoparticles by Bacillus subtilis RSB64 and process parameters optimization using central composite design. The two well-separated scattering spectra showing absorption peaks at 540 nm and 750 nm indicate the presence of anisotropic gold nanoparticles and the results were corroborated by transmission electron microscopy analysis. The presence of gold nanoparticles was further confirmed by energy dispersive X-ray studies. The functional groups responsible for the stability of gold nanoparticles were predicted by Fourier transform infrared spectroscopy. The gold nanoparticles biosynthesis were collective effects of three experimental process parameters viz pH, temperature and precursor concentration. These three parameters were statistically optimized wherein pH 11.0, substrate concentration 1:1 (v/v) and temperature of 50 °C resulted in the synthesis of stable flower shaped gold nanoparticles of 50 nm size. The results indicated the tailored biosynthesis of gold nanoparticles with a flower like morphology by multi process parameter analysis to finalize robust conditions for the synthesis using B. subtilis RSB64. These gold nanoflowers demonstrate increased surface area efficiency/reactivity and could be employed for sustained and controlled delivery of drugs.