Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications

提高时间聚焦多光子显微镜在神经生物学应用中的穿透深度

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Abstract

The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 µm in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications.

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