Abstract
Animal behavior can be modulated by multiple interacting factors. In rodents such as rats, these factors include, among others, the female estrous cycle, exposure to a novel environment, and light. Here, we used the open field test to disassociate differences in behavior resulting from each of these factors by testing the hypothesis that locomotor and anxiety-related behaviors differ between estrous cycle phases in female rats and that novelty and light exposure concurrently influence these behaviors in both female and male rats. Adult female rats were tested twice under red or white light in estrus and diestrus estrous cycle phases. Adult male rats were also tested twice under either red or white light. In females, an interaction between novelty and estrous cycle phase influenced locomotor and anxiety-related behaviors. In males, novelty influenced locomotor and anxiety-related behaviors differentially under red and white light. Light exposure increased anxiety-related behaviors in both males and females, but reduced locomotor behavior only in females. These findings reveal the complexities of behavioral testing and highlight the importance of factors such as the estrous cycle, novelty, and light exposure.