Abstract
The effect of elevated atmospheric CO(2) concentration [CO(2) ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) from six chambers with ambient [CO(2) ] (approximately 400 ppm) and six chambers with elevated [CO(2) ] (700 ppm). The V4 region of the 16S rRNA gene was PCR-amplified from the extracted DNA and sequenced on an Illumina MiSeq instrument. Above-ground plant biomass was not affected by elevated [CO(2) ] at anthesis, but plants exposed to elevated [CO(2) ] had significantly higher grain yield. The composition of the rhizosphere prokaryotic communities was very similar under ambient and elevated [CO(2) ]. The dominant taxa were Bacteroidetes, Actinobacteria, Alpha-, Gamma-, and Betaproteobacteria. Elevated [CO(2) ] resulted in lower prokaryotic diversity in the rhizosphere, but did not cause a significant difference in community structure.