Abstract
BACKGROUND: Ferns contain natural products with potential therapeutic applications. Current knowledge of the pharmacological properties of ferns, specifically those growing at high altitudes, is limited. This study aimed to evaluate the phytochemical contents as well as antibacterial, anti-glucosidase, and antioxidant activities of four highland ferns in Malaysia. RESULTS: Aqueous extracts of the leaves and rhizomes of Cyathea latebrosa, Dicranopteris curranii, Gleichenia truncata, and Phymatopteris triloba were analysed. P. triloba leaf extract had the highest contents of total flavonoids (118.6 mg/g dry matter), hydroxycinnamic acids (69.7 mg/g dry matter), and proanthocyanidins (29.4 mg/g dry matter). P. triloba leaf and rhizome extracts as well as G. truncata leaf extract inhibited the growth of both Gram-positive and Gram-negative bacteria. P. triloba leaf extract produced a minimum inhibitory concentration (MIC) value of 0.78 mg dry matter/mL when tested against Pseudomonas aeruginosa, which is 2.5-fold higher than that of ampicillin. Among all extracts, P. triloba leaf extract had the highest anti-glucosidase activity (EC(50) = 56 μg dry matter/mL) and also the highest antioxidant potential based on DPPH radical scavenging and Ferric Reducing Antioxidant Power assays. Antioxidant activities of both the leaf and rhizome extracts correlated positively with total flavonoid and hydroxycinnamic acid contents (R(2) = 0.80-0.95). On the other hand, anti-glucosidase activity correlated with total proanthocyanidin contents in both the leaf and rhizome extracts (R(2) = 0.62-0.84). CONCLUSIONS: In conclusion, highland ferns are potential sources of antibacterial agents, glucosidase inhibitors, and antioxidants.