Abstract
Three undescribed 9,10-seco-isopimarane diterpenoids, marginols I-K, and an unprecedent isopimara-8(9),15-diene diterpene, 14-epi-boesenberol F, together with a known 9,10-seco-isopimarane diterpenoid, kaemgalangol A, were isolated from the rhizomes of Vietnamese Kaempferia marginata. Marginols I and J contained a naturally very rare 6-oxabicyclo[3.2.1]octane-5-ol ring, while marginol K had a naturally rare oxepan-2-one ring in its structure. The unprecedented structures were elucidated by spectroscopic techniques, including HR-ESI-TOF-MS, UV, IR, and 1D and 2D NMR. The absolute configurations of marginols I-K and 14-epi-boesenberol F were determined by ECD calculations. The NO production inhibitory assay revealed that the isolated compounds, except marginol J, exhibited NO inhibitory activities with IC50 values ranging from 65.06 to 87.70 μM against lipopolysaccharide (LPS)-induced RAW264.7 cells.