Extended application of BACs-on-Beads technique in prenatal diagnosis

BAC-on-Beads技术在产前诊断中的扩展应用

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Abstract

INTRODUCTION: This study explored the application of bacterial artificial chromosomes (BACs)-on-Beads (BoBs) technique, especially its ability to detect microdeletion/microduplication regions with a single probe. METHODS: Both chromosome karyotyping and BoBs technique were applied on a total of 2218 pregnant women. Chromosome microarray analysis (CMA) was performed on patients whose cells were reported as being abnormal by BoBs technique with a single probe. RESULTS: Twenty-two cases were detected as microdeletion/microduplication with a single probe, which was consistent with the CMA results. CONCLUSIONS: We believe that the microdeletion/microduplication results detected by BoBs technique with a single probe provide comprehensive guidance for prenatal diagnosis.

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