Abstract
Polychlorinated biphenyls (PCBs) can be metabolized to hydroxylated polychlorinated biphenyls (OH-PCBs) as reported in a number of animal studies. However, there are few studies on OH-PCBs in vivo in whole plants. In order to explore the formation of OH-PCBs in whole plants in detail, poplars (Populus deltoides×nigra, DN34) were exposed to 3,3',4,4'-tetrachlorobiphenyl (CB77) in hydroponic solution. Poplars are widely used in phytoremediation applications and the complete genome has been sequenced. In this research, a HPLC-MS method was developed to directly determine the hydroxylated metabolites of CB77 (OH-CB77s), avoiding the experimental errors introduced by derivatization pretreatments required by gas chromatography-based methods. Three potential hydroxylated metabolites of CB77, including 6-hydroxy-3,3',4,4'-tetrachlorobiphenyl (6OH-CB77), 5-hydroxy-3,3',4,4'-tetrachlorobiphenyl (5OH-CB77) and 4'-hydroxy-3,3',4,5'-tetrachlorobiphenyl (4'OH-CB79), were determined in poplar tissues. The major product, 6OH-CB77, was detected in the roots, bottom bark, bottom wood, middle bark and middle wood for the whole poplar plants, but the minor product, 5OH-CB77, was detected only in the poplar roots. The concentration of 6OH-CB77 was about 10 times greater than that of 5OH-CB77 in the roots. However, the major mammalian metabolite, 4'OH-CB79 was not detected in any of the samples. The results suggest that the hydroxylated metabolic pathway of CB77 is via an epoxide intermediate in poplar.