Abstract
BACKGROUND: Cancer is a deadly and multifaceted disease that poses a significant challenge to treatment due to its heterogeneity and ability to adapt and evolve. Despite advancements in research and medicine, the development of effective treatment options remains a major obstacle in the battle against cancer. Manganese oxide (MnO) and iron (III) oxide (Fe(2)O(3)) nanoparticles (NPs) are increasingly used for numerous new applications in modern industrial sectors. However, the toxic and treatment impact of MnO and Fe(2)O(3) NPs has not been clearly elucidated on human cell lines at the cellular and molecular levels. OBJECTIVES: This study aimed to assess the potential cytotoxic effect of combining infrared (IR) laser therapy with MnO and Fe(2)O(3) nanoparticles on breast and colorectal cancer cells for cancer treatment. METHODS: We treated the cancer cells with MnO and Fe(2)O(3) NPs and then exposed them to IR radiation for 6, 12, 24, 48, and 72 hours to investigate the effectiveness of this cancer treatment approach. To evaluate cytotoxicity, we conducted assessments on Skbr3 and HT29 cancer cells, both individually and in combination, using various methods. RESULTS: The findings indicate that despite the inherent toxicity of NPs and IR laser radiation on cancer cells, the utilization of MnO and Fe(2)O(3) NPs in conjunction with IR laser radiation treatment had the highest cytotoxic impact on cancer cells. CONCLUSIONS: These findings suggest that using MnO and Fe(2)O(3) NPs in combination with IR laser therapy has great potential as an effective method for reducing the population of cancer cells.This revision maintains the original content while ensuring clarity and adherence to the AMA style guidelines.