Abstract
A microfluorometric assay using chlorotetracycline (CTC) as a probe for membrane-associated Ca(2+) in intact cotton (Gossypium hirsutum L. cv Acala SJ-2) root hairs indicated displacement of Ca(2+) by Na(+) from membrane sites with increasing levels of NaCl (0 to 250 millimolar). K(+)((86)Rb) efflux increased dramatically at high salinity. An increase in external Ca(2+) concentration (10 millimolar) mitigated both responses. Other cations and mannitol, which did not affect Ca(2+)-CTC chelation properties, were found to have no effect on Ca(2+)-CTC fluorescence, indicating a Na(+)-specific effect. Reduction of Ca(2+)-CTC fluorescence by ethyleneglycol-bis-(beta-aminoethyl ether) N,N'-tetraacetic acid, which does not cross membranes, provided an indication that reduction by Na(+) of Ca(2+)-CTC fluorescence may be occurring primarily at the plasmalemma. The findings support prior proposals that Ca(2+) protects membranes from adverse effects of Na(+) thereby maintaining membrane integrity and minimizing leakage of cytosolic K(+).