Abstract
In this study, electrochemical nanosensors were developed from the synthesized metal oxide (MO) nanoparticles by supporting it on a gold electrode (Au). The activity of the developed nanosensor toward the detection of malaria biomarker (β-hematin) was determined and the optimum conditions at which the maximum detection and quantification occurred were established. β-Hematin current response at the sensors was higher when compared with the bare Au electrode and followed the order Au-CuO (C) > Au-CuO (M) > Au-Fe(2)O3 (M) > Au-Fe(2)O3 (C) > Au-Al(2)O3 (M) > Au-Al(2)O3 (C) > bare Au. The developed sensors were stable with a relatively low current drop (10.61-17.35 %) in the analyte. Au-CuO sensor had the best performance toward the biomarker and quantitatively detected P. berghei in infected mice's serum samples at 3.60-4.8 mM and P. falciparum in human blood serum samples at 0.65-1.35 mM concentration.