A simple manual rosetting method for absolute CD4+ lymphocyte counting in resource-limited countries

一种适用于资源匮乏国家的简易手动玫瑰花结法进行绝对CD4+淋巴细胞计数

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Abstract

A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.

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