Abstract
OBJECTIVES: Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies. MATERIALS AND METHODS: In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol: water (1:1 v/v) extracts of A. biennis Willd. on two cancer human cell lines (K562 and HL-60) and J774 as normal cells. RESULTS: CH(2)Cl(2) extract was found to have the highest anti-proliferative effect on cancer cells. IC(50) values obtained in AlamarBlue(®) assay for CH(2)Cl(2) extract were 64.86 and 54.31 µg/ml on K562 and HL-60 cells respectively. In flow cytometry histogram of the cells treated with CH(2)Cl(2) extract, sub-G1 peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CH(2)Cl(2) extract after 48 hr contact with cells. CONCLUSION: The results can corroborate the cytotoxic and apoptotic effects of the CH(2)Cl(2) extract of A. biennis on the K562 and HL-60 cancer cell lines.