Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures

多层透明质酸基水凝胶配方适用于癌症基质细胞共培养的自动化 3D 高通量药物筛选

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作者:Brian J Engel, Pamela E Constantinou, Lindsey K Sablatura, Nathaniel J Doty, Daniel D Carson, Mary C Farach-Carson, Daniel A Harrington, Thomas I Zarembinski

Abstract

Validation of a high-throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.

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