Abstract
Health effects caused by ionizing radiations raise considerable concern among general public and radiation workers. To estimate ability of personal protective equipment (PPE) materials that reduce toxic effects of ionizing radiations, we developed an experimental bioassay system using Chinese Hamster V79 cells. The system developed here distinguished the biological effectiveness of X-ray that was significantly affected by elements composed of shielding materials. Survival of the cells exposed to sub-lethal dose of X-ray was enhanced more than 2 times when the X-ray was filtrated by a lead plate. Also filtration of the X-ray with a tungsten plate enhanced the cell survival more than three times. These results suggested the colony assay system developed here was useful for examination of the biological effectiveness of X-ray and the ability of PPE materials reducing the toxic effects caused by ionizing radiations.