Abstract
Understanding the molecular mechanisms regulating the development of bovine pre-implantation embryos and formation of embryo-like structures (blastoids) is essential to uncover the causes of infertility and develop promising novel assisted reproductive technologies (ARTs). This review presents an updated view of functional genome characterization of bovine pre-implantation development. The use of genomic phenotyping and candidate gene perturbation approaches to uncover molecular factors governing bovine early embryonic development are discussed. This review also delves into the latest breakthroughs in the development of bovine blastoids and highlights key molecular signaling for the creation of bovine blastoids.