Abstract
The present report communicates the genome sequence of a multidrug-resistant Staphylococcus hominis strain P1-NS, recovered from a nasal swab of a healthy pig in Bareilly, Uttar Pradesh, India. Initially misidentified as methicillin-resistant Staphylococcus aureus by phenotypic and PCR-based assays, whole genome sequencing clarified species identity as Staphylococcus hominis, emphasising the importance of genome-based diagnostics in antimicrobial resistance surveillance. The draft genome assembly consisted of 264 contigs of ≥ 200 bp with total genome assembly length of 2,199,316 bp, N50 of 137,700 bp, and GC content of 31.5%. A total of 2195 protein coding genes, 15 rRNA genes, and 70 tRNA genes, and multiple antimicrobial resistance determinants were present in draft genome assembly. This genomic information enhances understanding of antimicrobial resistance in livestock-associated coagulase-negative staphylococci in India. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-026-04766-3.