Abstract
In the present study, the effect of coffee leaf extract (CLE) on in vitro enzyme inhibition was studied. Furthermore, its impact on the high-fat diet (HFD)-induced obese mice (C57BL/6) at the levels of 100 and 200 mg/kg body weight along with positive control (orlistat) and the normal group maintained with starch-fed diet (SFD) was observed. CLE had significant α amylase and lipase enzyme inhibitory properties. In HFD-induced obese mice, treatment with CLE significantly reduced the body weight gain. The investigation demonstrated that CLE administration lowered blood glucose, total cholesterol, total triglycerides and LDL levels while increasing the HDL levels. It reduced the development of fatty liver by reducing hepatic fat accumulation and decreased the fat cell size in the adipose tissue. Further, CLE significantly increased the liver antioxidant enzyme activities and lowered the levels of hepatotoxicity markers in the serum when compared to the HFD-fed mice. The treatment also downregulated the mRNA expression of lipogenic transcription factors (SREBP-1c, CEBP-α) and enzymes (ACC, FAS) than HFD. Overall, the results indicate that coffee leaves have anti-obesity potential and can be used as functional ingredients in the development of innovative products for managing lifestyle disorders such as obesity.