Abstract
The optimization of extraction, chemical characterization, and the evaluation of antioxidant activity and α-amylase inhibition capacities of the cell wall polysaccharides extracted from Aspergillus niger ATCC 1004 were studied in this paper. The response surface methodology through a factorial design of three levels indicated the optimal conditions for extraction: pH 13 and 180 min. Characterization results showed that the polysaccharide is glucogalactan, consisting of β-D-galactose-linked units (1 → 6) and β-D-linked glucose (1 → 3). The antioxidant activity was evaluated through three in vitro assays. It could effectively scavenge DPPH, ABTS and hydroxyl radicals with inhibition rates of 82.12%, 75.87% and 79.24, respectively, at 6.4 mg/mL, which were higher than those of the other polysaccharides. For inhibitory activity against α-amylase, a blocking effect of 53.7% was observed at a concentration of 2 mg/mL. Therefore, the cell wall polysaccharides of Aspergillus niger, (1 → 3)(1 → 6)-β-D-glucogalactan, seem to be a promising source for use as an antioxidant, in addition to holding an in vitro hypoglycemic potential.