Abstract
In this study, the induction of esterase activity during the degradation of a high concentration of di(2-ethylhexyl) phthalate (DEHP) (1500 mg l(-1)) by Fusarium culmorum was investigated using Ca(NO(3))(2) as nitrogen source under liquid fermentation conditions. Assessments of esterase activities through biochemical tests and zymographic assays, as well as fungal growth were studied. A high concentration of DEHP increased esterase activity in F. culmorum, which produces five esterase isoforms (26.4, 31.7, 43, 73.6 and 125 kDa), which were different in abundance and molecular weight to those produced constitutively in glucose-containing medium (control medium). F. culmorum showed higher µ and Y (X/S) values in DEHP-containing medium than those observed in the control medium. F. culmorum has great potential for use in the restoration of sites contaminated with high concentrations of DEHP and even of other phthalates with less complex structures.