Abstract
The tolerance of the pentose-fermenting yeast Meyerozyma guilliermondii to the inhibitors released after the biomass hydrolysis, such as acetic acid and furfural, was surveyed. We first verified the effects of acetic acid and cell concentrations and initial pH on the growth of a M. guilliermondii strain in a semi-synthetic medium containing acetic acid as the sole carbon source. Second, the single and combined effects of furfural, acetic acid, and sugars (xylose, arabinose, and glucose) on the sugar uptake, cell growth, and ethanol production were also analysed. Growth inhibition occurred in concentrations higher than 10.5 g l(-1) acetic acid and initial pH 3.5. The maximum specific growth rate (µ) was 0.023 h(-1) and the saturation constant (ks) was 0.75 g l(-1) acetic acid. Initial cell concentration also influenced µ. Acetic acid (initial concentration 5 g l(-1)) was co-consumed with sugars even in the presence of 20 mg l(-1) furfural without inhibition to the yeast growth. The yeast grew and fermented sugars in a sugar-based medium with acetic acid and furfural in concentrations much higher than those usually found in hemicellulosic hydrolysates.