Abstract
Two-step bioleaching was applied using a cyanogenic bacterium Pseudomonas balearica SAE1 to recover gold (Au) and silver (Ag) from the computer printed circuit boards (CPCBs) via central composite design of a response surface methodology (CCD-RSM). To enhance Au and Ag recovery, factors like pH level, pulp density, temperature and glycine concentration were optimized and their interactions were studied. CCD-RSM optimization resulted in 73.9 and 41.6% dissolution of Au and Ag, respectively, at initial pH 8.6, pulp density 5 g/L, temperature 31.2 °C, and glycine concentration 6.8 g/L, respectively. Two quadratic models were proposed by RSM which can be utilized as an efficient tool to predict Au and Ag recovery through bioleaching. The experimental results are in line with the predicted results, indicating reliability of RSM model in enhancing the Au and Ag recovery from CPCBs. The increased bioleaching yield of Au and Ag from discarded CPCBs has its importance in industrial e-waste recycling and safe disposal.