Abstract
Coptis herbs, which are important herbal medicinal materials, are the dried rhizomes of various plants. In China's herbal market, drying herbs can severely influence morphological markers such as shape, color, and odor, which make consumers difficult to precisely identify the herbs and effectively evaluate the quality. Here, we present the phylogenetic analysis of four Coptis herbal medicinal materials based on internal transcribed spacer sequences. C. chinensis, C. omeiensis, C. deltoidea and C. teeta constituted a monophyletic group. In this group, C. omeiensis and C. deltoidea cluster together, and they form the sister group with C. teeta, and C. chinensis locate the outermost of C. omeiensis, C. deltoidea and C. teeta. At the same time, the HPLC-DAD method was applied to simultaneously analyze main alkaloids from seventeen different samples. On the basis of the robust phylogenetic topology, the parsimony reconstructions of six effective medicinal constituents were implemented to elucidate the evolutionary history in Coptis herbs, and thus evaluate the herbal quality. The results showed that C. chinensis had been considered to be the best herb, not only the high content in single constituent but also in total alkaloids. In addition, all the samples from seventeen habitats were considered as qualified herbs, and they could meet the requirements of national quality standards for Coptis herbs in China.