Abstract
Fluorescence is an innovative technique that has captivated scholars in recent years due to its superior sensitivity and selectivity. The development of microfluidic components has added to its appeal, particularly given the technology ability to control fluid using very small quantities (microliter range) and achieve high liquid throughput. We have combined these two technologies to develop a lab-constructed simple system for measuring fluorescence, notable for the following features:•The device constructed entirely in our lab and programmed for measuring the fluorescence of liquids using microfluidic technology, delivered excellent results. The regression coefficient R² (0.9995) was obtained five points between 0.001-0.01µg .ml(-1). Moreover, the reproducibility standard deviation (%) of 0.008 µg .ml(-1) fluorescein dye remained at zero, for ten repeated experiments.•The device was full automated using a smartphone as a data logger, and lab-constructed programs.•The results were satisfactory with a detection limit of 1 × 10(-4) µg.ml(-1). This proposed system can measure over 200 samples per hour making it highly efficient and eco-friendly due to the reduced use of reagents and lower waste production. The fully automated system can effectively be used to determine fluorescein dye concentrations. Another application (micro pump view) manages all actions required in this microfluidic system, such as operating the two lab-constructed peristaltic pumps.