Abstract
The aim was to describe a methodology developed to study the relationship among the spatio-temporal patterns of habitat utilization, feeding ecology and microplastics (MPs) contamination across the different ontogenetic phases of fishes belonging to different trophic levels and living along the riverine-estuarine-coastal food web. The Goiana Estuary's water column was examined for the seasonal and spatial variation of MPs and their quantification relative to zooplankton, demersal fish species contamination following the same sampling design. The density of MPs in the water column determines their bioavailability. Interest in studies on MPs distribution in relation to spatial and temporal variation of environmental factors and fauna are increasing in quantity and quality. If the ecological strategies presented in this study were replicated in other estuary, comparisons could be made in order to describe how ecosystems work. Standard protocols for sampling, extraction, enumeration and classification of MPs and others pollutant ingested by fishes have been developed and are presented here to encourage comparisons. Standardized and comparable sampling designs and laboratory procedures are an important strategy in order to devise and transfer managerial solutions among different sites and comparisons along time when studying the same environment.