Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway

车前草苷通过RAS/MEK/ERK通路调控慢性粒细胞白血病细胞K562的增殖、凋亡和分化

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作者：Bingjie Zhao, Hong Che, Linlin Li, Lian Hu, Wenjing Yi, Li Xiao, Songshan Liu, Zhufa Hou

期刊：

Heliyon

影响因子：

3.400

时间：

2023

起止号：

2023 Dec 16;10(1):e23580.

doi：

10.1016/j.heliyon.2023.e23580

研究方向：

信号转导、肿瘤

疾病类型：

白血病

细胞类型：

粒细胞

信号通路：

Apoptosis、MAPK/ERK

Conclusion

ASP inhibited the proliferation, promoted apoptosis and differentiation of K562 cells by regulating the RAS/MEK/ERK pathway, and played a good anti-CML role.

Methods

The IC50 of ASP in K562 cells was calculated by the concentration-effect curve. Cell viability, apoptosis, and differentiation were detected by CCK8, flow cytometry, benzidine staining, and WB analysis, respectively. Further, RNA-seq was used to analyze the possible mechanism of ASP regulating K562 cells.

Objective

This study aimed to explore the effects and possible mechanisms of ASP on the biological behavior of K562 cells based on RNA-seq. Materials and

Results

ASP significantly inhibited the proliferation, and promoted apoptosis and differentiation of K562 cells. A total of 117 differentially expressed genes were screened by RNA-seq, mainly involved in the RAS/MEK/ERK pathway. PD98059 was used to inhibit the RAS/MEK/ERK pathway in K562 cells, and results confirmed that PD98059 could not only inhibit the RAS/MEK/ERK pathway, but also inhibit the regulation of ASP on the proliferation and differentiation of K562 cells.