Abstract
BACKGROUND: Increasing concern has been expressed about the declining sperm count of humans and the potential fertility effects of clomiphene citrate, a synthetic oestrogen-antagonist on human reproductive health in the last few decades. This study aims to investigate the influence of cashew nut supplemented diet on fertility activity of clomiphene citrate in male rats. METHODS: The rats were divided into six groups n = 6: rats fed basal diet; rats fed basal diet and then given clomiphene citrate (cc) orally; rats fed diet supplemented with 10% processed cashew nut and given cc orally; and rat fed diet supplemented with 20% processed cashew nut and given cc orally; rats fed diet supplemented with 10% processed cashew nut and rat fed diet supplemented with 20% processed cashew nut for fourteen days. RESULTS: The results revealed that there was a significant (p < 0.05) improvement of total testosterone level and epididymal sperm count, viability and progressive motility in all the groups, in comparison to control, with more significance in combination therapy groups. Similarly, increased testicular and epididymal total antioxidant capacity (TAC), nitric oxide (NO), total thiol (T-SH), non protein thiol (NPSH) levels, Glutathione-S-transferase (GST) and steroidogenic enzymes activities with concomitant decrease in reactive oxygen species (ROS) level, thiobarbituric acid reactive substances (TBARS) production and arginase activity, as well as non-observable histopathologic changes in the testes were observed in all the groups when compared with control, with more significance in combination therapy groups. CONCLUSION: Therefore, this present study shows that the combination of cashew nut supplemented diet and clomiphene citrate could modulate vital biomolecules associated with male reproductive function. Thus, this finding supports the concept that the combination therapy of cashew nut and clomiphene citrate may be used to treat male partners suffering from infertility.