MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

MagC,用于体积相关光学和电子显微镜的超薄切片磁性集合

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作者:Thomas Templier

Abstract

The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

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