Abstract
Xenia effect significantly influences fruit quality, with underexplored impacts on pear aroma. This study systematically evaluated eight cultivars' effects on 'Starkrimson' pears using electronic nose (E-nose), headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). Pollination with 'Zaosu' (ZS) pear maximized volatile organic compound (VOC) concentrations (terpenoids, esters, aldehydes) and achieved optimal solid-acid ratio (291.78), enhancing fruity, green, and sweet aromas. Conversely, 'Huagai' (HG) pear pollination reduced VOC accumulation and fruit quality. Other cultivars showed intermediate effects on total soluble solids (TSS), titratable acidity (TA), and aroma. GC-MS identified 14 key aroma-active compounds, while GC-IMS detected four critical small-molecule VOCs, demonstrating methodological complementarity. Xenia-driven pollen-parent effects regulated VOC biosynthesis through lipoxygenase (LOX) and mevalonate (MVA) pathways, enhancing aroma complexity. ZS pollination optimized 'Starkrimson' market value and consumer preference, providing actionable strategies for horticultural optimization through cultivar selection.