Abstract
Molecular-level understanding of plasma cell (PC) differentiation has been modeled using lipopolysaccharide (LPS) stimulation in vitro. However, this system does not involve the B-cell receptor (BCR)-a critical component of B cell biology. Here, we present a protocol for in vitro PC differentiation system dependent on BCR signaling that easily scales up for cell number-demanding applications, including protein complex purification. We describe how to set up this system and detail applications for endogenous complex purification of chromatin-associated proteins. For further details on the use and execution of this protocol, please refer to Sciammas et al. (2011) and Ochiai et al. (2018, 2020).
Keywords:
Antibody; Cell Biology; Cell Differentiation; Chromatin immunoprecipitation (ChIP); Flow Cytometry/Mass Cytometry; Gene Expression; Immunology; Mass Spectrometry; Molecular Biology.