Abstract
The water strider Limnogonus fossarum fossarum (F.) (Hemiptera: Gerridae) shows a macropterous, micropterous, and apterous polymorphism. Although a long photoperiod condition induces winged morphs, preliminary studies have revealed that crossing between winged morphs increased the proportion of macropterous individuals, suggesting that the genetic factors also affect wing-morph determination in this species. Assessing the genetic backgrounds of wing polymorphism requires constant and repeatable methods for rearing. This study attempts to establish a continuous rearing method for L. f. fossarum under constant diet conditions. Initially, we maintain the water striders with two Drosophila species as a food, but viability until adulthood is less than 20%. We then add the storage pest Plodia interpunctella (Hübner), which are readily reared in the laboratory, to the diets. As a result, nymphs fed on P. interpunctella (even only until the second instar) show significantly higher viability and shorter developmental period than nymphs fed on Drosophila alone. Moreover, feeding on D. melanogaster (Meigen) reared on cholesterol-enriched medium instead of a normal medium significantly increases viability in the next generation. This means that only the two food-insect species are enough for establishing a substantial number of individuals in segregating generations (F2 and backcross), limiting DNA and RNA contaminations from food insects with genome information. Thus, the present rearing method opens the way to elucidating the genetic backgrounds of the wing polymorphism in L. f. fossarum.