Abstract
Emerging contaminants such as sunscreens, hair dyes and flame retardants have been found at important concentrations in surface water (river, lake, ocean), but their negative impact on different aquatic species is not fully known. This study evaluated the effect of benzophenone (BZ), 2,5-diaminotoluene sulfate (PTD), p-phenylenediamine (PPD) and tetrabromobisphenol A (TBPA) on survival (LC50) and the impact of sublethal concentrations (LC25) on the activity of enzymes linked to stress oxidative process in brine shrimp under two temperature conditions (22 °C and 28 °C) for 24 h and 48 h of exposure time. LC50 values obtained for each chemical substance and the activity of GST, AChE and LDH were significantly affected by the temperature conditions and exposure time. In contrast, GPx was only altered by the tested compound. TBBPA (LC50 from 17.05 up to 28.55 μg/L) and BZ (LC50 from 14.86 up to 24.49 mg/L) resulted in the most toxic substances for A. salina. The impact of dyes, such as PTD and PPD, on aquatic organisms is limited. These are the first results that show that not only dyes, but their respective by-products induce harmful effects in brine shrimp (LC50 for PTD and PPD were 23.6-396.3 and 52.0-164.9 mg/L respectively). Although this study model was very useful to evaluate the ecotoxicity of the different ECs, additional research is needed to increase available information related to the effects of dyes and other non-studied micropollutants on aquatic systems in general.