Abstract
Two single-tube media, lactose-tryptone-lauryl sulphate broth (LTLSB) and lauryl-tryptose-mannitol broth were tested in parallel in a series of 1111 tests on water samples from a variety of sources. LTLSB was found to be superior both in gas and indole production from Escherichia coli when incubated at 44 degrees C and is recommended as the most suitable single-tube confirmatory test for this organism.