Abstract
Zoysiagrass stands out as a crucial native turfgrass due to its exceptional abiotic stress tolerance, extensive adaptability, and high ornamental value. In this study, we generated a high-quality chromosome-level genome assembly of Compadre (COM) zoysiagrass, leveraging PacBio SMRT sequencing and Hi-C scaffolding technologies. The resulting genome assembly (312.42 Mb) is anchored on 20 chromosomes, with a Scaffold N50 of 18.72 Mb. In total, 49,074 genes and 306,768 repeat sequences were annotated in the assembled genome. The first chromosome-scale genome of Zoysia japonica 'Compadre' provides a critical genetic resource for cold-tolerant turfgrass breeding through identifying stress-responsive candidate genes. Additionally, we have successfully established a cell nucleus extraction and library construction protocol tailored for zoysiagrass ATAC-seq technology, and a total of 80 low temperature tolerance candidate genes were preliminarily identified via ATAC-seq and RNA-seq profiling, thereby initiating the exploration of turfgrass epigenomics.