Abstract
Kidney and spleen antigens from cow, sheep, goat, European moose, reindeer, deer and roe deer were prepared by boiling and ethanol precipitation and tested against rabbit and caprine anti-bovine kidney sera. Two different levels of antigen concentration were used for immunizing animals in the various groups. In the group using high antigen concentrations, the precipitation reaction obtained initially disappeared after further inoculations, probably due to immunological tolerance. Sera tested from animals inoculated with low level antigen concentration showed a variation in the immunological response. The caprine antibovine sera showed distinct species specific reaction, while rabbit antisera showed either species specific or organ specific reaction, with a limited degree of cross reaction. In the production of species specific antisera against ruminant tissue antigens, goat seems to be preferable to rabbit.