A Glutathione-Consuming Bimetallic Nano-Bomb with the Combination of Photothermal and Chemodynamic Therapy for Tumors: An in vivo and in vitro Study

一种利用光热疗法和化学动力疗法联合治疗肿瘤的谷胱甘肽消耗型双金属纳米炸弹:一项体内和体外研究

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Abstract

BACKGROUND: Chemodynamic therapy (CDT) faces challenges of low catalytic ion efficiency and ROS production. We developed a ROS nano-bomb, Cu/ZIF-8@GA-Fe, to address these issues. METHODS: The nano-bomb was synthesized by doping copper into ZIF-8 and assembling Fe(3+) and gallic acid (GA). It was tested for reactive oxygen species (ROS) generation in acidic conditions and its photothermal properties. RESULTS: In an acidic micro environment, Cu/ZIF-8@GA-Fe effectively released Fe(3+) and Cu(2+), depleting GSH and generating ROS. The GA-Fe coating provided photothermal heat and was used to enhance Fenton reactions via dual ions for increasing ROS production. In vivo and in vitro experiments, Cu/ZIF-8@GA-Fe inhibited tumor growth with minimal side effects. CONCLUSION: Cu/ZIF-8@GA-Fe shows promise for safe and effective CDT, offering a synergistic approach to tumor therapy.

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