Theranostic Angiopep-2-Conjugated FeTaO(x)@Au Core-Shell Magnetic Nanoparticles for Glioma Treatment and Dual Medical Imaging

用于胶质瘤治疗和双重医学成像的诊疗一体化Angiopep-2偶联FeTaO(x)@Au核壳磁性纳米粒子

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Abstract

In this work, iron-tantalum oxide nanoparticles (FeTaO(x) NPs) with a gold coating modified by Angiopep-2 (FeTaO(x)@Au-ANG) were developed to achieve dual-modality imaging and magnetically induced hyperthermia therapy for glioma. The 13.5 nm sized FeTaO(x)@Au-ANG NPs' exhibited superparamagnetic behavior with excellent dual T(1)/T(2)-weighted MRI contrast of Fe existence and enhanced X-ray attenuation for computed tomography (CT) imaging, enabling accurate tumor localization through complementary imaging modalities. The incorporation of Ta and Au not only improved biocompatibility but also provided a high CT contrast effect. Upon magnetic stimulation, the NPs efficiently elevated the intratumoral temperature, leading to a significant (∼90%) reduction in glioma cell viability. ANG modification further enhanced the targeted uptake of NPs by glioma cells. Immunohistochemical analysis revealed extensive coagulative and glial necrosis, elevated GFAP expression, and a reduced Ki67 index, consistent with effective tumor ablation. In vivo, FeTaO(x)@Au-ANG NPs treatment markedly suppressed tumor growth and extended survival by 18 days. Overall, this multifunctional nanoplatform demonstrates synergistic MRI/CT imaging-guided magnetic hyperthermia with high therapeutic efficacy and minimal side effects, offering strong potential for clinical cancer theranostics.

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