Abstract
Between 2007-2008 and 2010-2012, 870 organ samples were collected from wild boars in 16 Transylvanian counties. Anaplasma phagocytophilum DNA was identified using a nested PCR protocol that amplifies a fragment of the 16S rRNA gene. Prevalence was compared between sampling periods and counties using Fisher's exact test. In total, 39 (4·48%) samples tested positive, with significantly higher values recorded in the second period, caused by an increased infection rate in boars from Sibiu county. Positive cases tended to concentrate in the central part of the country. During the second sampling period, A. phagocytophilum was detected in two additional counties, suggesting a spatial spreading of the pathogen. The results confirm that Transylvanian wild boars are naturally infected with A. phagocytophilum, thus raising awareness concerning a potential zoonotic cycle. This is the first study to evaluate spatial and temporal variations of A. phagocytophilum distribution in wild boar populations from Transylvania.