Abstract
In this work, we investigated the influence of silver nanoparticle (AgNP) size (diameters of 20, 50, and 100 nm) and magnetic bead (MB) size (diameters from 100 to 4500 nm) on silver-gold galvanic exchange signal generation in magnetic electrochemical assays. Two conjugation strategies, including biotin-streptavidin interaction and a streptavidin-specific aptamer interaction, were compared to assess differences in binding chemistry and conjugation efficiency. Calibration studies showed that 50 nm diameter AgNPs provided the best sensitivity and galvanic exchange efficiency, yielding the lowest detection limits across both conjugation strategies. Larger AgNPs produced stronger signals but reached saturation rapidly, whereas smaller particles required higher concentrations to achieve equivalent silver content. Among MBs, 1000 nm beads consistently gave the highest galvanic exchange efficiency, offering sufficient surface area for AgNP loading while minimizing steric hindrance and electrode obstruction. These findings were confirmed by complementary electrochemical impedance spectroscopy, UV-Vis absorbance, and SEM imaging, which collectively demonstrated the strong influence of bead size on charge transfer resistance and conjugation efficiency. Overall, the combination of 50 nm AgNPs with 1000 nm MBs emerged as the optimal configuration, providing improved sensitivity and reproducibility. We believe these results offer valuable design guidelines for the development of next-generation aptamer-based electrochemical biosensors for biomarker detection.