Abstract
Copper contamination in the environment poses significant risks to both soil and human health, making the need for reliable monitoring methods crucial. In this study, we report the use of the EmStat Pico module as potentiostat to develop a portable electrochemical biosensor for copper detection, utilizing yeast Saccharomyces cerevisiae cells immobilized on a polydopamine (PDA)-coated screen-printed electrode (SPE). By optimizing the sensor design with a horizontal assembly and the volume reduction in the electrolyte solution, we achieved a 10-fold increase in current density with higher range of copper concentrations (0-300 µM CuSO(4)) compared to traditional (or previous) vertical dipping setups. Additionally, the use of genetically engineered copper-responsive yeast cells further improved sensor performance, with the recombinant strain showing a 1.7-fold increase in current density over the wild-type strain. The biosensor demonstrated excellent reproducibility (R(2) > 0.95) and linearity over a broad range of copper concentrations, making it suitable for precise quantitative analysis. To further enhance portability and usability, a Bluetooth-enabled electrochemical platform was integrated with a web application for real-time data analysis, enabling on-site monitoring and providing a reliable, cost-effective tool for copper detection in real world settings. This system offers a promising solution for addressing the growing need for efficient environmental monitoring, especially in agriculture.