Abstract
In this article, we present the design, fabrication and characterization of a microfluidic device and a dedicated electronic system to perform 8 multiplexed electrochemical measurements of synthetic miRNA strands, as well as the biochemical protocols developed for the functionalization of the electrodes and the quantification experiments. The outcomes of this work highlight that the parallelization of eight microchannels containing 2-electrode cells driven by the dedicated electronics offers a solution as robust as a conventional 3-electrode cell and commercially available potentiostats. In addition, this solution presents the advantage of simultaneously reduce the microfabrication complexity, as well as offering an integrated; multiplexed and portable system for the quantification of miRNA. The results presented demonstrate that the system shows a linear response on concentrations down to 10(-18) mol/L of perfect matched reporter and capture sequences of synthetic miRNA.