Background
The carcinogenic role of lncRNA LOXL1-AS1 in thoracic aortic aneurysm has been confirmed, but its role in the pathogenesis of periodontitis remains unclear. Our preliminary deep sequencing data revealed LOXL1-AS1 downregulation in periodontitis and its inverse correlation with IL-1β, a critical inflammatory mediator in periodontitis. This study was therefore performed to investigate the potential interaction between LOXL1-AS1 and IL-1β in periodontitis.
Conclusion
This study is the first to report LOXL1-AS1 downregulation in periodontitis. Moreover, LOXL1-AS1 might inhibit the proliferation of periodontitis-affected PDLSCs and downregulate IL-1β to improve periodontitis.
Methods
The study included 30 periodontitis patients (18 males and 12 females at the age of 34 to 44 years, with a mean of 39.3 ± 2.1 years) and 30 healthy controls (18 males and 12 females at the age of 33 to 44 years with a mean of 39.2 ± 2.0 years). The effects of LOXL1-AS1 overexpression on IL-1β were evaluated by RT-qPCR and Western blot. CCK-8 assay was used to analyze cell proliferation.
Results
LOXL1-AS1 was downregulated in periodontitis-affected periodontal ligament stem cells (PDLSCs) compared with healthy PDLSCs, while IL-1β was upregulated in periodontitis-affected PDLSCs and was inversely correlated with LOXL1-AS1. LOXL1-AS1 overexpression mediated IL-1β downregulation in PDLSCs. IL-1β treatment did not affect LOXL1-AS1 expression. Moreover, LOXL1-AS1 overexpression inhibited the proliferation of periodontitis-affected PDLSCs. LOXL1-AS1 overexpression and IL-1β knockdown increased Bax/Bcl-2 ratio and caspase-3 level.