Association of salivary C-reactive protein with the obesity measures and markers in children

Increased level of salivary CRP in children may be considered as a non-invasive marker for childhood obesity for detection of the risk factors for the development of metabolic dysregulation.

Seventy-six children (40 normal weight and 36 overweight/obese) were recruited for this study. Body weight, height, and waist circumference measurement were obtained. The saliva sample was collected from all the participants. According to the Center for Disease Control and Prevention (CDC), the participants were classified into the normal weight or overweight/obese depending upon the body mass index (BMI) percentile ranking. The obesity panel of salivary markers resistin, C-C Motif Chemokine Ligand 2 (CCL2)/monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), complement factor D, and interleukin-10 (IL-10) were determined using human magnetic Luminex performance assay. The receiver operating characteristics (ROC) analysis was used to determine the area under the curve (AUC) to identify the best salivary biomarker in children. Linear regression and Pearson's correlation analyses to determine the association between the parameters.

Overweight and obesity is a pro-inflammatory state. This study aimed to examine the level of the salivary obesity markers in normal weight (NW) and overweight/obese (OW/OB) children, association with the obesity measures and the interrelations between the biomarkers. Subjects and

The obesity biomarkers resistin, MCP-1, TNF-α, IL-6 and CRP were significantly high in overweight/obese compared to normal weight. Salivary CRP (AUC: 0.866, 95% CI: 0.780-0.952; p<0.0001) showed superiority area under ROC curves with good discriminatory power than resistin, MCP-1, TNF-α, and IL-6. BMI z-score, WC z-score, and WHtR z-score showed a significant association (p<0.0001) with CRP. The CRP significantly (p<0.0001) correlated with resistin, CCL2/MCP-1, TNF-α, IL-6, and IL-10 by linear regression and Pearson's correlation analysis.