Abstract
BACKGROUND: This study sought to examine the mechanism of the Jian Pi Tiao Gan Yin in the treatment of obesity by network pharmacology. METHODS: The active components and corresponding targets of the Jian Pi Tiao Gan Yin were identified using the traditional Chinese medicine systems pharmacology database and analysis platform, and the obesity-related targets were acquired from the Online Mendelian Inheritance in Man database. The drug and disease targets were also identified. Cytoscape software was used to construct the "active component target" network diagram. The protein-protein interaction network was drawn using the Search Tool for the Retrieval of Interacting Genes/Proteins platform, and the Cytoscape MCODE plugin was used to find clusters for the protein cluster analysis. The gene annotation and analysis were performed with the Metascape database via functional databases, such as the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and Autodock and PyMOL were used for the molecular docking. RESULTS: The GO analysis identified 244 target genes of the Jian Pi Tiao Gan Yin, 1,378 targets of obesity, and 123 targets of drug and disease. Additionally, 208 biological process items, 38 molecular function items, and 33 cell component items were also identified. The KEGG pathway analysis identified the hypoxia-inducible factor, forkhead box O, cyclic adenosine monophosphate, and vascular endothelial growth factor signaling pathways. The results of the molecular docking showed that the main active components of the Jian Pi Tiao Gan Yin in the treatment of obesity were quercetin, kaempferol, stigmasterol, luteolin, isorhamnetin, β-sitosterol, sapogenin, tanshinone, and formononetin, all of which have been proven to bind to core obesity-related proteins, such as AKT1, interleukin-6 (IL-6), vascular endothelial growth factor A (VEGFA), tumor necrosis factor (TNF), tumor protein 53 (TP53), prostaglandin-endoperoxide synthase 2 (PTGS2), caspase-3 (CASP3), mitogen-activated protein kinase 1 (MAPK1), JUN, and epidermal growth factor (EGF). Thus, our study revealed the potential mechanism of the Jian Pi Tiao Gan Yin as a multi-component, multi-target, and multi-channel treatment for obesity. These findings lay the foundation for further studies on the mechanism of the Jian Pi Tiao Gan Yin in obesity treatment. CONCLUSIONS: The Jian Pi Tiao Gan Yin can be used as a multi-component, multi-target, and multi-channel treatment for obesity.