Discussion
The expression of galectin-1 and PIBF proteins in equine trophoblast and the marked difference in lifespan between the endometrial cups in intraspecies horse versus interspecies mule pregnancies support a likely role for these two proteins protecting the fetal trophoblast from maternal immune attack and/or modulation of the invasiveness of endometrial cup cells.
Results
Galectin-1 stained the non-invasive trophoblast between Days 15 and 20 but thereafter stained only the invasive trophoblast cells of the chorionic girdle, both before and after they invaded the endometrium to form the endometrial cups. PIBF, on the other hand, stained both the invasive and non-invasive trophoblast throughout the period of gestation studied. Of particular interest was the relative lack of staining of the endometrial cup cells in mule compared to horse pregnancies for galectin-1 and PIBF prior to the earlier and more rapid death and desquamation of the mule cup cells.